ABSTRACT
Objective To investigate the effect of low oxygen tension (7.5% O2) on human embryo development in vitro and outcome of women undergoing IVF-ET. Methods The data of 280 routine IVF cycles with fresh embryo transfer on day3 conducted in the 2nd People's Hospital of Yunnan Province from February 2012 to August 2013 were retrospectively analyzed. Two groups, 7.5%O2 low oxygen tension (n=120) and 20%O2 high oxygen tension (n=160),were setup for early embryo (D1-D3) development in vitro. Results Good quality embryo on day 3 in low oxygen tension group showed morphologically appearance of blastomere mutually clinging to each other,a characteristic of slight compaction. Higher rate of good quality embryo (66.22%V. 47.54%) and of clinical pregnancy (53.33%v. 39.22%) were found in the low oxygen tension group. There were no significantly differences in rates of fertilization,cleavage and embryo utilization respectively between the two groups ( all>0. 05) . Conclusion High quality early stage human embryos can be developed in vitro in low oxygen tension, and may improve clinic outcome of IVF.
ABSTRACT
Objective To study the effect of mental intervention on pregnant result in patients undergoing IVF-ET treatment.Methods 206 patients undergoing IVF-ET treatment in the hospital from February 2012 to Jan-uary 2013 were selected and randomly divided into two groups. The experiment group (n=103) was followed by mental intervention after the patients enrolled the IVF-ET treatment, the control group (n=103) enrolled the IVF-ET treatment without mental intervention.Results The HAMA and HAMD quantity grades for anxious and depression of patients in the experiment group were significantly lower than those of control ( <0.01), and the pregnancy rate of experiment group (45.63%) was higher than that of control (30.10%) <0.05. Conclusion Mental intervention for patients undergoing IVF-ET treatment could alleviate their anxiety-depression and improve the pregnancy rate of IVF.